Composite

Part:BBa_K3781202:Design

Designed by: Nicolas Bayer   Group: iGEM21_TU_Kaiserslautern   (2021-10-15)


L1:_3xHA_RBD_Strep8His, MocloMania Composite


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 619
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 619
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 557
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 619
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 619
    Illegal NgoMIV site found at 705
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 729


Design Notes

This composite part was assembled from three basic parts that are part of the MocloMania collection. Due to the highly standardizes set of overhangs that all our basic parts comply with, assembly could be achieved by a simple Moclo ligation. Assembling this part helped us to verify the basic parts' correct adaptation towards the employed MoClo cloning standard. Furthermore, immunostaining against the fused 3xHA tag allowed for evaluation of recombinant protein expression via western blot and thus provided important input about the progress of our research success as well as on the effectiveness of our purification procedures.


Source

For information on the origin of the genetic sequences present in this composite part, please consult the individual basic part pages.